ASCP Molecular Biology (MB) Technologist Exam 2025 – 400 Free Practice Questions to Pass the Exam

The statement is true because dot blot is indeed a solid support hybridization technique. In dot blot, nucleic acid samples are applied directly onto a solid support, such as a membrane or filter, in discrete dots. Following this, the bound nucleic acids can be hybridized with labeled probes. This methodology allows for the detection of specific sequences among multiple samples simultaneously.

The solid support provides a stable platform for the hybridization process, enabling effective washing and probing steps that are key to obtaining specific and sensitive results. The use of solid supports is a hallmark of various hybridization techniques, and dot blot exemplifies this by allowing the immobilization of nucleic acids for further analysis without the need for gel separation.